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* ПИННИ / 107 / -- Москва --
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Vadimpynny

13.05.2005 21:19

URL #1

Фирма "ПИННИ" предлагает:
I. Секвенирование ДНК и ПЦР-амплификатов на ABI Prism GA 3100 и 3730 ( Цена за 1 определение – 350-450 руб. Сроки выполнения – 3-4 дня). Выделение и очистка ДНК.
II. Генетический паспорт. Спортивная генетика. Генотипирование и генодиагностика. Генетические тесты для животных. Анализ нуклеотидных полиморфизмов ДНК человека (точечных мутаций (SNP), делеций, инсерций). Стоимость 1 анализа SNP - 250 рублей (подробности на http//www.pynny.ru).
III. Геномика и транскриптомика. Изучение экспресcии генов.
Создание и анализ ДНК-микро- и макроматриц (DNA-chip):
подбор и синтез праймеров;
амплификация ДНК (полноразмерных ДНК-матриц).
микронанесение ДНК на мембраны или стекло (до 15х384 образцов одновременно);
тестирование матрицы;
Дополнительно:
сканирование ДНК-макроматриц после гибридизации;
перенос колоний (picking) с чашек на платформы с 384-лунками;
анализ ДНК-матриц.
IV. Генная инженерия. Клонирование и экспрессия генов (E.coli, эукариоты). Создание рекомбинантных плазмид, получение штаммов продуцентов рекомбинантных белков, очистка (SDS-PAGE, HPLC, FPLC), анализ (масс-спектрометрия MALDI-TOF, LC-ESI MS).
Микробиологические работы. Исследование пролиферации клеток, а также их метаболизма с нативным и рекомбинантным ДНК (геномное сканирование и протеомный анализ).
V. Протеомика.Идентификация белков (в растворе или в геле) по массам их трипсинолизных фрагментов, используя базу данных NCBI;
Сиквенс пептидов методом электроспрейной ионизации (ESI MS/MSn) или MALDI TOF/TOF;
Одно- и двумерный электрофорез белков (SDS- и 2D-PAGE);
SDS-PAGE белков с их идентификацией методом HPLC, сопряженной с MS (SDS-PAGE + LC-ESI MS/MS);
VI. Масс-спектрометрический (MS) анализ химических соединений, липидов, пептидов, белков и олигонуклеотидов (MALDI-TOF, LC-ESI MS).

/ Companies, #16594 /

107023, Москва, ул. Малая Семеновская, д. 3А, стр. 2

Tel.: +7 (499) 713-50-60, 255-28-46, 526-70-08, 246-44-38

Fax: +7 (499) 713-50-60

E-mail: pynnygen@mail.ru, pynnygen1@mail.ru

Web address: http://www.pynny.ru/

Specialization: Nucleic Acid Purification, Microarrays, Gene engineering, Sequencing, Oligonucleotides, Peptides, Protein expression, Purification, Custom microarrays, Chromatograpy, Microscopy, Tests, Analyses, Diagnostics, Other Bio Services

Geography of the work: Russia

This post has been edited by Redactor: 16.10.2012 09:10

guest: replicawatches
IP-stamp: frTsmmXT1Bdb6
guest

24.04.2021 15:54

URL #2

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watchesbiz
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02.06.2021 11:21

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watchesbiz
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25.09.2021 06:07

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fashionicon
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02.03.2022 16:36

URL #7

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13.03.2022 22:11

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Monika Singh
IP-stamp: frvxpRxMGRuPg
guest

10.11.2022 07:55

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Monika Singh
IP-stamp: frvxpRxMGRuPg
guest

10.11.2022 08:40

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Helenwatcher
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15.11.2022 05:49

URL #13

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