Родные, подскажите, какой предпочтительно использовать
Protease Inhibitor Cocktail для очистки Histidine-tagged proteins из колей?

Я предпочитаю от Roche EDTA-free Это сильно зависит от того как Вы клетки давить будете, ультразвуком или прессом. Если звучать, то можно и Сигму. Все равно добавляю PMSF, оно для этого нет серьезных причин - привычка

сигмовский без едта

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Ничего кроме PMSFа не добавляю, да и то как та монашка, береженого бог бережет. В общем случае для очистки очистки Histidine-tagged proteins из колей протеазные ингибиторы вообще не особо нужны. А ЕДТА там дейстиветельно совсем не к месту

"В общем случае для очистки очистки Хистидине-таггед протеинс из колей протеазные ингибиторы вообще не особо нужны"
Ето собственные данные или теория???


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Если он колийные белки в колях экспрессирует, да если это хорошие белки... то не нужны.

Это практика. Проблем с протеолизом при лизисе колей почти не бывает. Где бывают проблемы с протеолизом, равно как и с синтезом полноразмерного продукта - во время роста и индукции культуры. Это раз. Если есть протеолиз при лизисе, то теоретическую проблему в колях могут представлять сериновые протеазы и металло-протеазы. Первые вполне удовлетворительно ингибируются PMSF (APMSF, Pefabloc для тех, кто работает медленно , они стабильнее). Металло-протеазы EDTA-free таблетками толком не ингибируются. Так за что вы там деньги платите? За красивые рекламные слова?

белок(и) был в растворимой фракции или в тельцах?

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Спасибо всем.
Белки у меня, как правило, растворимые, рушу звуком.
Последний раз использовал таблетки роше с EDTA, перед посадкой на смолу скомпенсировал EDTA избытком MgCl2. Вроде все нормальненько получилось. Может это самый праильный вариант и есть?
Но таблетки у меня эти все равно кончаются, хочу новые. Роше подходит не очень, просто потому что из Москвы покупать не шибко удобно.

Airpods are wireless Bluetooth ear buds. The AirPods contain a microphone that filters out background noise as well as built-in accelerometers capable of detecting taps and pinches which enables automatic pausing of audio.For more information click on this link How to connect your AirPods to a computer ? and get information

Let us know How to boost Wi-Fi signal in home? Wifi signal connects your device for internet connections. When signal is low or your data is fully used you can use wifi connection because it provides you unlimited internet data. It also provides mobile to mobile internet connections called Hotspot. Wifi allow users to enjoy unlimited data.

A hyperlink or link is a type of text that is created by HTML. As we all know that a website is made up of many pages and all these pages are connected to each other only through HTML links.So today we will show you How to create a Hyperlink in HTML? get information

Gmail is a free email service, which means that you can easily send any message sitting at home to any person. You can also send photos, documents, files by attaching them with the message.For more information, you can get How to Sign out Gmail in Mobile? information by clicking on this link

Excel is being used in the field of calculation. To keep so much data and to keep so many files safe, Excel is being used in which you can create Spreadsheet.So for more information What is Spreadsheet in Excel? click on this link

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How many parts of Computer? There are several types of computers and each type of computer has a set of unique features. The first is the internal parts of the computer and the second is the external parts of the computer.

Microphone is an input device that converts your voice into digital data. With the help of microphone, you can put audio data in the computer.then click on this What is Microphone? link and go

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GIF is the best way to say everything without saying anything. GIFs are usually short scenes from popular TV shows and movies, which are turned into mini-video snippets. But How to make a gif with a video? And which are the best free GIF makers? We’ll show you everything you need to know.

Google Docs gives you the freedom to create a wide range of documents, from essays to newsletters. It is also equipped with different features for formatting to ensure your final product looks good, including the ability to add bullet points. This may have you wondering How to indent bullet points in google doc? go to by clicking on this link

In this article covers How to move a column in excel? , cut and paste a column. There are several ways to rearrange the columns in an Excel worksheet using the mouse, command and drag and drop but one is easier than all the others.

In this how to article How to embed photos in google contact? You have been using Google Photos to backup your photos to the cloud but Google offers no option for you to embed the uploaded pictures on to your website.

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On your Mac you need to convert HEIC to JPG. you can read this article. HEIC is apple’s proprietary version of the HEIF or High-Efficiency Image File format. This newer file format is intended to be a better way to save your pictures, making your images smaller in terms of data while retaining high quality. HEIC is better than JPG. HEIC is a new image format, built to be more efficient than existing file types. It stands for High-Efficiency Image Container, and it’s a variation of HEIF (High-Efficiency Image File Format).Click on this link How to convert HEIC to JPG on a Mac? and go

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Google Sheets API allows you to write values and formulas to cells, ranges, sets of ranges, and entire sheets. Functions can only be inserted through the cells feed. In this article we will show you How to enter function googlesheets api? You can see an example below section. how exactly enter function googlesheet api.

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What are alphanumeric characters ? The term ‘Alphanumeric’ refers to a collection of alphabetic characters (A-Z) and numerals (0-9). It is also called as ‘Alphameric’ and is useful to define a group of Latin letters and Arabic numbers. So that, a text built from this collection and for storing text and website addresses.

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The miles to km formula is used to convert the unit of a quantity given in miles to kilometers. Miles to km are the units of length. They are used to measure the distance from one point to the other point. These two measures are mostly used to measure geographical land areas. To convert miles to km, the conversion factor is required. It means that 1 mile is approximately equal to 1.60934 km. Let us learn about the miles to km formula with few solved examples at the end.

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