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Окраска сердца после инфаркта phthalocyanine blue

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666, 16.09.2009 00:38
Подскажите, пожалуйста, как правильно проводить окраску сердца крыс после инфаркта краской phthalocyanine blue. По методике нужно вводить в полость левого желудочка сердца 1 мл краски, подождать какое-то время, пока краска проциркулирует, омоет, прокрасит сердце, а потом вырезать его.
Я ввожу краску в левый желудочек, сердце бъется, кровь циркулирует, краска распространяется по сосудам (даже видно, что сосуды языка становятся синими), жду около 3х минут, потом вырезаю сердце. Почему-то неишемизированные участки сердца не синеют, краска их не прокрашивает. Причем, есть абсолютная уверенность, что инфаркт был сделан хорошо. Может, есть какие-то тонкости/хитрости окрашивания сердца phthalocyanine blue? Очень жду советов!! confused.gif
VVolkov, 16.09.2009 00:54
А методику не приведёте полностью и цели работы. Спасибо. Что можно, конечно.
guest: ммм , 16.09.2009 09:51
Классический фталоцианининовый голубой - это пигмент, нерастворимый не только в воде, но и в большинстве органических растворителей.

Те если это не химически модифицированный фталоцианин(так чтобы сделать его водорастворимым), то распространение красителя по кровеносной ситеме возможно только в виде коллоида. А если коллоид, то очень много в возможности проникновения красителя в тот или иной орган, зависит от среднего размера частиц.

А в остальном присоединясь к Волкову.
Heckfy, 16.09.2009 21:33
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