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pravda li, chto...

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Anonymous, 15.10.2002 16:41
...меркаптоетанол настолько едкий, что будучи пролившис из пробирки в центрифужный ротор неминуемо ведет к заржавению ротора и полной негодности???
Ниче не могу найти по этому поводу )-:
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Anonymous, 15.10.2002 17:42
вернее буфер с меркаптоетанолом, пролившис из пробирки...
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Dimych, 15.10.2002 18:19
Не правда, во всяком случае если используются обычные концентрации. Но роторы после себя мыть все равно надо, даже если там меркаптоэтанола вовсе не водилось. Ротору от ультры не обязательно скорродировать на треть, чтобы развалиться на ходу. А они, поверьте мне, разваливаются! Но мыть надо любые роторы, буде они загажены, равно как и прочие рабочие инструменты. smile.gif
То есть я имел ввиду, что немедленной коррозии не произойдет.
[ 15-10-2002: Сообщение отредактировано: Dimych ]
Tom1, 15.10.2002 19:01
Правда...
Ротор как правильно было замечено мыть надо....
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gregTS, 15.10.2002 19:17
И горазды же вы прикалываться. Может у человека горе, а они..
Tom1, 15.10.2002 19:34
Какие приколы какая разница чем ротор уделать. Мыть то всегда надо...
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Dimych, 15.10.2002 19:44
gregTS
Какие уж приколы... Роторы действительно рвутся иногда на высоких оборотах. Или Вы полагаете, что фирмы в качестве крышек на ультрах полуторасантиметровую сталь для красоты используют? На моей памяти один такой случай был. Лично не присутствовал (чему рад), но центрифугу после этого надо было реставрировать (смена камеры, крышки и вала). Так что лучше считать, что корродирует и заблуждаться, чем угробить ротор и прибор из простой небрежности.
Anonymous, 15.10.2002 19:44
Ну да, мыть надо, кто ж знал, что из пробирки с завинчиваюсчейся крышкой и заполненной до половины в угловом роторе эта дрянь может просочится. Шча коллеги уголовное дело заводют на меня за порчу оборудования (шутка)
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Dimych, 15.10.2002 19:50
wild type mutant
Ыыыыы!!!! Дык у тебя пробирка лопнула! За поликарбонатными пробирками это водится. Очень внимательно надо смотреть трещинки около дна. Хотя в некоторых случаях ты их все равно не увидишь. Не расстраивайтесь, это не катастрофа, если только образец не сверхценный. Имелось ввиду, что загрязнения нельзя оставлять в роторе.
Tom1, 15.10.2002 23:55
На будусчее никогда не фуговать органику в поликарбонатных пробирках.
Они ее не держат.
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Anonymous, 16.10.2002 17:33
Какая органика - тиоцианатный буфер для выделения тотальной РНК? Ну, все равно, видно эти пробирки никуда не годятся для центрифугирования. а стеклянные брать, они ж без крышек, туда может РНКаз насыпаться и усио пропало, шеу, усио пропало...
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Tom1, 16.10.2002 18:42
Зачем сразу стеклянные???
Просто надо использовать полипропиленовые пробирки вот и все.
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Anonymous, 17.10.2002 14:01
Да флакончики то и были полипропиленовые, только, наверно малость переполнила я их, поетому и пролилось.
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serg, 17.10.2002 15:37
Дурацкий вопрос - а под крышками, что на пробирки навинчиваются, прокладки резиновые имеются?
Anonymous, 17.10.2002 18:27
Нет, нету прокладок, это такие 45 мл флаконы с зелеными крышечками, бывают есчо с синими по 50 мл - но те для центрифугирования не годятся.
А центрифуга не ультра, на ней max можно 10 тыс г крутить.
а вот на крышке от ротора имеется резиновая прокладка, но она растянулас в кишку и болтается, в обсчем только для вида что она имеется.
Ну сегодня опять крутила, налила совсем понемногу во флаконы, ничего не пролилос. Наверно, все таки 25 мл было замного для этих флаконов в угловой ротор.
Ну да ладно, один раз случилась осечка, просто дурацки себя чувствуеш, когда тебе нечинают втирать с глазами по пятаку, что меркаптоетанол, однако, яд такой, что разйедает железо ( прямо не меркаптоетанол а хуже какой ядреной кислоты кислоты) :-Д
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Tom1, 17.10.2002 18:55
Это сильно с зеленэкими крышечками....
Вообсчето странно если обсчи обьем 45 мл то 25мл это много -так не бывает не важно какои ротор угловои или бакет.
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Dimych, 17.10.2002 22:47
25 не должно было протечь и без всякой крышки. До 2/3 нормально все держится, если ротор не уродский. Странно. Вы засуньте в ротор пробирку и прикиньте по вертикали, докуда наливать можно.
yack, 18.10.2002 17:39
Вообще по теме мое мнение - Sorvall must die, Beckman rulez forever! У Сорвала течет почти все, если налить чуть больше двух третей
avor, 18.10.2002 17:41
А вот железо в растворе с низкой концентрацией меркаптоэтанола должно корродировать лучше чем в воде, из-за того, что склонно образовывать сульфиды. Однако роторы делают из люминя - это раз, во-второых по сравнению с солянкой или разбавленной азоткой МЭ - это детский лепет, в-третьих даже чтобы в солянке что-то существенно растворилось нужно время, ну хотя-бы сутки. И в-четвертых МЭ довольно летуч и может испариться раньше чем испортит оборудование(кроме всего прочего он восстановитель и в высоких концентрациях будет защищать железо от коррозии), а ГТЦ, не проверял, но из общих соображений, должен ингибировать коррозию.
Ну, а вообще, лучше не проливать, а если пролил немедленно убрать. А МЭ не так страшен, как его малюют.
Anonymous, 18.10.2002 19:53
согласен с Avor
Dimych, 19.10.2002 01:31
Чессказать, люминевому ротору для низкооборотных фуг коррозия по барабану. Разве сто эстетику портит. Хотя еще пробирки могут застревать... Ну если тольно щелочь концентрированную внутря набузырить, тогда он серьезно проржавеет. А можно просто слегка соленой водички и пару медных гаек в каждую дырку (из инструкции начинающему вредителю).
Я изначально подумал, что об ультрах разговор. Тогда бы была понятна взволнованность мутантовых коллег.
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If your constant use of the snooze สล็อตเว็บตรงแตกง่ายไม่ล็อคยูส button and your morning zombie routine is getting old, there’s help. It begins ทางเข้าnigoal with figuring out the different reasons 123 why you can’t wake up in the morning and what to do baccarat about them. Chances are you’re not getting สูตรบาคาร่า enough sleep and need to tweak your เว็บตรงไม่ ล็อค ยู ส ไม่มี ขั้นต่ำ bedtime routine. If a sleep disorder or other PG underlying condition is to blame for your morning QR Code sleepiness, there are treatments หวย available. Going to bed and waking up at the same time every day is a must if you ลิงค์รับทรัพย์ want to get on a good sleep schedule and train yourself to wake up early.
123goal 123goal, 02.12.2022 07:37
Caffeine is a stimulant - after all, that’s สล็อตเว็บตรงแตกง่ายไม่ล็อคยูส exactly why we drink it! But drinking coffee and other ทางเข้าnigoal caffeinated drinks later in the day could be 123 causing you to take longer to fall baccarat asleep on an evening. Where possible, try to สูตรบาคาร่า avoid caffeine in the afternoon, and เว็บตรงไม่ ล็อค ยู ส ไม่มี ขั้นต่ำ especially in the evening; use it as your wake PG up aid in the morning. If you’re stuck in the QR Code habit of having a hot drink in the หวย afternoon. If you are reliant on coffee to keep you going through the day then it’s probably a good idea to cut down ลิงค์รับทรัพย์ your intake so that you’re not relying on it to keep you awake.
guest: 123vega , 02.12.2022 13:16
The air is warm and Superslot เว็บตรง buttery as workers knead PG Slot เครดิตฟรี 50 บาท mounds of raisin-flecked dough สล็อต เครดิตฟรี 100 ไม่ต้องฝาก inside the Kexerei bakery in Dresden. This is the busiest Superslot เครดิตฟรี 50 ยืนยันเบอร์ ใหม่ล่าสุด 2022 time of year but, like every other business and household in the superslot เครดิตฟรี country, the firm's bills are going up, diminishing the festive PG Slot Wallet เว็บตรง cheer. "Wages have increased drastically, energy costs have risen, as have PG Slot เว็บตรง the prices for our ingredients - butter has doubled in price," says owner PG Slot ฝาก-ถอน ไม่มีขั้นต่ำ Matthias Walther. "I can't pass all that on to the customers." It's an issue ทดลองเล่นสล็อต PG that preoccupies the German government too. Wary of the impact on the economy, it's spending around
123goal 123goal, 03.12.2022 11:45
Learning how to let go of someone you love is the สล็อตเว็บตรงแตกง่ายไม่ล็อคยูส only way you can be the architect of your own life. It’s also ทางเข้าnigoal one of the most challenging things 123 you’ll ever have to do. Once you baccarat overcome that fear, you’ll สูตรบาคาร่า feel free. You’ll feel relieved. You’ll know เว็บตรงไม่ ล็อค ยู ส ไม่มี ขั้นต่ำ that you have the inner strength to PG conquer anything. And you can QR Code begin to forge your own path to fulfillment. Knowing you หวย need to let go and actually letting go are two very ลิงค์รับทรัพย์ different things. These tips will help you discover how to move on once and for all.
123goal 123goal, 05.12.2022 09:49
Fever is usually associated with สล็อตเว็บตรง แตกง่าย ไม่มี ขั้นต่ำ physical discomfort, and most สล็อต เครดิตฟรี 30 ไม่ต้องฝากก่อน ไม่ต้องแชร์ ยืนยันเบอร์โทรศัพท์ people feel better when a fever is treated. But depending 123GOAL on your age, physical condition, and the บาคาร่าออนไลน์ underlying cause of your fever, สูตรบาคาร่าฟรี you may or may not require pg สล็อตเว็บตรง medical treatment for the fever alone. Many experts believe that โปรโมชั่น fever is a natural bodily defense against บาคาร่า infection. There are also many non-infectious สล็อต โจ๊ก เกอร์ เว็บตรงไม่ผ่านเอเย่นต์ ไม่มี ขั้นต่ำ causes of fever. Treatments vary depending on the cause of the fever. For example, antibiotics ลิงค์รับทรัพย์ would be used for a bacterial infection such as strep throat.
123goal 123goal, 06.12.2022 12:03
Leaving any relationship is difficult. Leaving a bad one isn’t สล็อตเว็บตรง แตกง่าย ไม่มี ขั้นต่ำ necessarily any easier. The shift from สล็อต เครดิตฟรี 30 ไม่ต้องฝากก่อน ไม่ต้องแชร์ ยืนยันเบอร์โทรศัพท์ powerless to empowered is a gentle one, but lies in 123GOAL the way you experience the relationship. It often บาคาร่าออนไลน์ takes as much resourcefulness, สูตรบาคาร่าฟรี energy and strength to stay in a bad pg สล็อตเว็บตรง relationship as it does to leave. With a shift in โปรโมชั่น mindset, experience and บาคาร่า expectation, the resources you use to stay สล็อต โจ๊ก เกอร์ เว็บตรงไม่ผ่านเอเย่นต์ ไม่มี ขั้นต่ำ and to blind out the seething hopelessness of it all ลิงค์รับทรัพย์ can be used to propel you forward.
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123goal 123goal, 07.12.2022 04:45
Dengue is the most widely distributed mosquito-borne สล็อตเว็บตรง แตกง่าย ไม่มี ขั้นต่ำ viral disease in the world. It continues to สล็อต เครดิตฟรี 30 ไม่ต้องฝากก่อน ไม่ต้องแชร์ ยืนยันเบอร์โทรศัพท์ spread rapidly because of climate 123GOAL change, rapid urbanization, and population growth. Outbreaks are บาคาร่าออนไลน์ usually seasonal, peaking สูตรบาคาร่าฟรี during and after rainy seasons. The World Health pg สล็อตเว็บตรง Organization classifies dengue as one of โปรโมชั่น the top ten threats to public health. Hospitalized บาคาร่า dengue patients must be closely สล็อต โจ๊ก เกอร์ เว็บตรงไม่ผ่านเอเย่นต์ ไม่มี ขั้นต่ำ observed and their fluid intake carefully managed, ลิงค์รับทรัพย์ according to strict guidelines. 
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