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"...1: Phys Rev Lett. 2007 Oct 5;99(14):148104. Epub 2007 Oct 5.Click here to read Links
Extension of a DNA molecule by local heating with a laser.
Ichikawa M, Ichikawa H, Yoshikawa K, Kimura Y.
Department of Physics, Graduate School of Sciences, Kyushu University, Fukuoka 812-8581, Japan.
Thermal convection and thermophoresis induced by mum-scale local heating are shown to elongate a single DNA molecule. An infrared laser used as a point heat source is converged into a dispersion solution of DNA molecules, which is observed under a fluorescent microscope. The thermal convection around the laser focus manifests as extensional flow for the long DNA chain. A simulation of thermal convection that reproduces the experimental condition provides numerical support for the stretching caused by thermal convection. This DNA elongation technique is a novel method for manipulating the intact single DNA molecules, and it can be applied to a "lab on a chip".
PMID: 17930727 [PubMed - in process]..."
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"...1: Phys Rev Lett. 2007 Oct 5;99(14):148104. Epub 2007 Oct 5.Click here to read Links
Extension of a DNA molecule by local heating with a laser.
Ichikawa M, Ichikawa H, Yoshikawa K, Kimura Y.
Department of Physics, Graduate School of Sciences, Kyushu University, Fukuoka 812-8581, Japan.
Thermal convection and thermophoresis induced by mum-scale local heating are shown to elongate a single DNA molecule. An infrared laser used as a point heat source is converged into a dispersion solution of DNA molecules, which is observed under a fluorescent microscope. The thermal convection around the laser focus manifests as extensional flow for the long DNA chain. A simulation of thermal convection that reproduces the experimental condition provides numerical support for the stretching caused by thermal convection. This DNA elongation technique is a novel method for manipulating the intact single DNA molecules, and it can be applied to a "lab on a chip".
PMID: 17930727 [PubMed - in process]..."
.. - PubMed
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- 45 - 450
All: 1
Review: 1
[Click to change filter selection through My NCBI.]
1: Am J Clin Dermatol. 2000 Nov-Dec;1(6):337-48.Links
The role of PUVA in the treatment of psoriasis. Photobiology issues related to skin cancer incidence.
Gasparro FP.
Photobiology Laboratory, Department of Dermatology & Cutaneous Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA. francis.gasparro@mail.tju.edu
Photochemotherapy with methoxsalen (8-methoxypsoralen) and long wavelength ultraviolet (UV) radiation (referred to as 'PUVA' for psoralen plus UVA) is commonly used to treat psoriasis and vitiligo. These vastly different diseases respond to the therapy by different mechanisms even though the immediate effects of the therapy--the photomodification of cellular biomolecules--is the same for each. Because psoriasis is not cured by PUVA, patients receive many treatments over their lifetime and have a significantly increased risk for the development of skin cancers (primarily squamous cell carcinomas). In this article the basic aspects of psoralen photobiology are reviewed briefly. Several recent studies describing the incidence of skin cancer in UVA treated psoriasis cohorts are comparatively reviewed. In addition the impact of the analysis of mutations in the tumor suppressor gene, p53, are summarized. An unexpected mutation spectrum (very few PUVA type T-->A transversions and frequent UVB solar signature C-->T transitions) suggest that effects other than direct DNA photoadduct formation may be at play. These analyses suggest that it may be possible to improve the therapeutic efficacy of PUVA by a careful evaluation of the mode of delivery. In this review the science behind PUVA is summarized. In addition, the incidence of skin cancer as a long term consequence of repeated treatments is surveyed. To relate clinical observations to molecular events, the nature of p53 mutations found in skin cancers from psoriasis patients is also analyzed. Finally some suggestions for improving the delivery of PUVA therapy are presented.
PMID: 11702610 [PubMed - indexed for MEDLINE]
-
- 45 - 450
All: 1
Review: 1
[Click to change filter selection through My NCBI.]
1: Am J Clin Dermatol. 2000 Nov-Dec;1(6):337-48.Links
The role of PUVA in the treatment of psoriasis. Photobiology issues related to skin cancer incidence.
Gasparro FP.
Photobiology Laboratory, Department of Dermatology & Cutaneous Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA. francis.gasparro@mail.tju.edu
Photochemotherapy with methoxsalen (8-methoxypsoralen) and long wavelength ultraviolet (UV) radiation (referred to as 'PUVA' for psoralen plus UVA) is commonly used to treat psoriasis and vitiligo. These vastly different diseases respond to the therapy by different mechanisms even though the immediate effects of the therapy--the photomodification of cellular biomolecules--is the same for each. Because psoriasis is not cured by PUVA, patients receive many treatments over their lifetime and have a significantly increased risk for the development of skin cancers (primarily squamous cell carcinomas). In this article the basic aspects of psoralen photobiology are reviewed briefly. Several recent studies describing the incidence of skin cancer in UVA treated psoriasis cohorts are comparatively reviewed. In addition the impact of the analysis of mutations in the tumor suppressor gene, p53, are summarized. An unexpected mutation spectrum (very few PUVA type T-->A transversions and frequent UVB solar signature C-->T transitions) suggest that effects other than direct DNA photoadduct formation may be at play. These analyses suggest that it may be possible to improve the therapeutic efficacy of PUVA by a careful evaluation of the mode of delivery. In this review the science behind PUVA is summarized. In addition, the incidence of skin cancer as a long term consequence of repeated treatments is surveyed. To relate clinical observations to molecular events, the nature of p53 mutations found in skin cancers from psoriasis patients is also analyzed. Finally some suggestions for improving the delivery of PUVA therapy are presented.
PMID: 11702610 [PubMed - indexed for MEDLINE]
Gypoteza, 24.10.2007 15:50
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As condensation of sugar phosphates and nitrogenous bases is thermodynamically unfavorable, these compounds, if ever formed, should have undergone rapid hydrolysis. Thus, formation of oligonucleotide-like structures could have happened only if and when these structures had some selective advantage over simpler compounds. It is well known that nitrogenous bases are powerful quenchers of UV quanta and effectively protect the pentose-phosphate backbones of RNA and DNA from UV cleavage. To check if such a protection could play a role in abiogenic evolution on the primordial Earth (in the absence of the UV-protecting ozone layer), we simulated, by using Monte Carlo approach, the formation of the first oligonucleotides under continuous UV illumination. The simulations confirmed that UV irradiation could have worked as a selective factor leading to a relative enrichment of the system in longer sugar-phosphate polymers carrying nitrogenous bases as UV-protectors. Partial funneling of the UV energy into the condensation reactions could provide a further boost for the oligomerization .
.
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As condensation of sugar phosphates and nitrogenous bases is thermodynamically unfavorable, these compounds, if ever formed, should have undergone rapid hydrolysis. Thus, formation of oligonucleotide-like structures could have happened only if and when these structures had some selective advantage over simpler compounds. It is well known that nitrogenous bases are powerful quenchers of UV quanta and effectively protect the pentose-phosphate backbones of RNA and DNA from UV cleavage. To check if such a protection could play a role in abiogenic evolution on the primordial Earth (in the absence of the UV-protecting ozone layer), we simulated, by using Monte Carlo approach, the formation of the first oligonucleotides under continuous UV illumination. The simulations confirmed that UV irradiation could have worked as a selective factor leading to a relative enrichment of the system in longer sugar-phosphate polymers carrying nitrogenous bases as UV-protectors. Partial funneling of the UV energy into the condensation reactions could provide a further boost for the oligomerization .
volmin, 07.04.2020 23:50
constant12, 10.04.2020 11:34
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vrida, 17.05.2020 09:32
vrida2, 11.07.2021 03:50
ok1231, 30.08.2021 11:28
ok1231, 30.08.2021 11:29
ok1231, 30.08.2021 11:30
guest: 123vega , 02.02.2022 08:22
Since it overthrew Aung San Suu Kyi's democratically elected government in a coup one year ago, Myanmar's military - known as the Tatmadaw - has gone on to shock the world by killing hundreds of its own civilians, including dozens.
guest: 123vega , 07.02.2022 08:17
Traders import unwanted garments mainly from Europe and the US - to resell locally and to other Latin American nations. But more than half of the 60,000 tonnes of clothes imported each year ends up in illegal desert landfills.
guest: 123vega , 09.02.2022 07:24
Nobel Peace Prize winner Malala Yousafzai has spoken out in support of six Indian students fighting for their right to wear the hijab in class. The teens have been protesting in Karnataka state for weeks, drawing attention to a college's ban.
guest: 123vega , 14.02.2022 08:27
Ukraine has called for a meeting with Russia and other members of a key European security group over the escalating tensions on its border. Foreign Minister Dmytro Kuleba said Russia had ignored formal requests to explain the build-up of troops.
guest: 123vega , 18.02.2022 08:21
Police in Ottawa have detained two leaders of the trucker convoy in the Canadian capital as they move to break up the last stronghold of the protests against the nation's Covid rules.Tamara Lich was arrested on Thursday evening.
guest: 123vega , 21.02.2022 12:15
Australia has reopened its international border for the first time in nearly two years, bringing joyful family reunions and a boost to tourism. The country imposed some of the world's strictest travel bans after shutting itself off in March 2020 due to Covid.
Guest, 26.02.2022 12:10
Just before the emergency EU leaders' meeting on Ukraine started, a Brussels official told us he thought this would be one of the toughest and most emotional EU summits ever. From what I heard went on behind closed doors, he wasn't wrong.
vrida2, 16.03.2022 06:19
Richard321, 16.03.2022 14:11
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guest: 123vega , 18.03.2022 10:50
The American space agency is rolling out its new giant Moon rocket for the first time. The vehicle, known as the Space Launch System (SLS), is being taken to the pad at the Kennedy Space Center in Florida to conduct a dummy countdown.
guest: 123vega , 21.03.2022 09:10
A few days after Russia's invasion of Ukraine, one of Russia's largest media companies, Russian Media Group (RMG), released a statement explaining why it would no longer be playing certain artists on its popular radio stations or music TV channel.
guest: 123vega , 30.03.2022 06:47
US Secretary of State Antony Blinken has denied that the United States has any plans to bring about regime change in Russia or anywhere else. Mr Blinken's comments come a day after President Joe Biden said his Russian counterpart, Vladimir Putin, should not be allowed to remain in power.
guest: 123vega , 04.04.2022 09:13
A team of Amazon workers has forced the technology giant to recognise a trade union in the US for the first time. Workers at a New York warehouse voted 55% in favour of joining the Amazon Labor Union. The group is led by former Amazon worker Chris Smalls, who made his name protesting against safety conditions at the retail giant during the pandemic.
3xfaz, 15.04.2022 18:12
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