Expression of Recombinant Genes in Eukaryotic Systems 
Methods in Enzymology кн.306
Ред. Glorioso J.C., Schmidt M.C.
Elsevier Science; 1999; 403стр.; ISBN: 0121822079
The ability to express recombinant proteins in eukaryotic cells has greatly increased our ability to study protein function in a variety of settings. Initial work in this field focused on gaining an understanding of the cis-acting sequences necessary for mRNA expression, processing, and translation. As the field has matured, the more sophisticated task of finely regulating gene expression has taken center stage. Researchers want to be able to limit expression to particular tissues and to determine the timing and level of expression. In addition, exciting new technologies are being developed that use small RNA molecules to regulate expression of endogenous genes. In this volume of Methods in Enzymology, we have brought together a number of exciting new techniques for regulated gene expression in eukaryotic cells.
We hope that this volume will prove to be a valuable resource for those who are looking for new methods to express recombinant proteins in eukaryotic systems. We realize that no single volume can be complete, and certainly there are methods for recombinant protein expression not represented here. However, we have tried to bring together many novel and creative methods currently being used in many top laboratories.