Methods in Molecular Biology кн.7
Ред. Murray, Edward J.
Humana Press; 1991; 456стр.; ISBN: 0896031780
Murray's new handbook on Gene Transfer and Expression Protocols sets forth both current and new methodologies in a clear, concise, easy-to-follow manner, following the successful formula of the classic volumes in Humana's Methods in Molecular Biology series. Each chapter is devoted to a thorough exposition of a single technique. An Introduction explains the significance of the protocol and provides background information. A Materials section lists all the requirements for the technique discussed. A Methods section details the procedure in a step-by-step protocol. A Notes section alerts the reader to pitfalls that may be encountered, as well as alternatives that may be used for successful completion of the experiment. Each technique is designed to guarantee optimum results. This volume is an outstanding new benchtop manual that provides protocols for introducing an isolated gene into a cell line, using various transfection techniques. Topics and techniques include: viral vectors - reporter genes - analysis of steady-state level of transcription - assay for newly initiated transcriptional complexes - immunocytological techniques - assay of structure and replication state of transfected genes - overall strategy for defining regulatory sequences Providing the latest techniques in a fast-paced area, Gene Transfer and Expression Protocols is an absolutely essential handbook for everyone involved in cytogenetics, cell genetics, molecular biology, and related fields.