Подскажите, пожалуйста, какие варианты векторов с индуцибельной экспрессией можно использовать, чтобы потом клеточки можно было уколоть мышкам и индуцировать экспрессию в мышках. Желательно, чтобы вся конструкция помещалась в одну плазмиду, так как клеточная линия уже имеет две стабильно трансфецированные плазмиды под бластицидином и пуромицином.
Эстроген-чувствительный промотор мне не подходит, так как клетки чувствительные к эстрогену. Тетрациклиновый промотор уже занят, это первая и вторая мои плазмиды.
А еще есть вопрос, можно ли скринировать клеточные клоны не по экспрессии белка, а по экспрессии фермента, отвечающего за устойчивость к пуромицину (если да, то посоветуйте антитела, пожалуйста)
Большое спасибо!
ship, 17.09.2009 13:30
(Parhit @ 17.09.2009 04:15)
А еще есть вопрос, можно ли скринировать клеточные клоны не по экспрессии белка, а по экспрессии фермента, отвечающего за устойчивость к пуромицину (если да, то посоветуйте антитела, пожалуйста) Большое спасибо!
А какой в этом смысл? А если клон пустышка и нет экспрессии нужного белка? или уровень низкий?
angel eyes The bad, 17.09.2009 19:46
cadmium
aivanov, 17.09.2009 21:13
металлотеининовый промотор
Parhit, 17.09.2009 22:10
а купить их можно? нужно что-то легкодоступное и используемое в литературе. Спасибо всем отозвавшимся!
Guest, 17.09.2009 22:11
cadmium -yes
Parhit, 18.09.2009 00:20
а ссылочку на страничку компании или просто название вектора можно?
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