Подскажите, пожалуйста, как можно запрограммировать прибор для ПЦР в реальном времени (Applied Biosystems 7500) для постановки обычной качественной ПЦР?

Качественной с использованием красок или просто хочется потом на геле смотреть?
Если первое, то, насколько я помню, в режиме Allelic Discrimination.
Второе в любом режиме.

Спасибо за ответ. У нас второй вариант, нужно просто посмотреть на геле. Проблема в том, что когда мы отключаем краски, то прибор выдаёт Error. Не могли бы Вы уточнить какие конкретно режимы нужно установить?

Ну включите краски, а красок не добавляйте. Прибор попросту не увидит увеличения концентрации, но вам же оно и не нужно.

или просто добавьте Рокс в знак уважения к умному прибору...

Постойте, а анализировать будете далее в агарозе?

когда у меня циклера простого не было так я и сиквенсы гонял - а что делать писал ему сибру, первый раз думал краски сдохнут, фигвам-так что с уверенностью заявляю что сибровый канал бигдай не ест

у меня были любители гонять обычные ПЦР в аби7000
пробирки подписывали на боковых стенках цветными фломастерами
после этого был Реал-Тайм вообще без образцов - блок отмывал неделю

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