Работаю с лимфоцитами человека. Какие housekeeping genes лучше всего использовать при проведении ПЦР для измерения относительной экспрессии. Хорош ли GAPDH? Спасибо

Смотря, что оцениваете РНК или ДНК.

"Оцениваем" РНК для эксрессии ... генов , наверное.
А что, можно и по ДНК?

А можно и проверить в своих условиях "свой" ХКГ

Хорош, конечно. Но лучше по двум сразу.

Оцениваем РНК. А какой еще ген посоветуете? Смотрим гены, продукты которых участвуют в апоптозе.

18SrRNA. Только не забудьте в RT кроме oligo(dT) (если с ними кДНКу делаете) добавить рендом праймеры. GAPDH нормально, но иногда тоже пляшет. Можно еще актин, тубулин и т.д. попробовать.

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