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4-тиоуридин

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mesentsev, 04.03.2013 18:19
Сколько может прожить в DMSO 200 mM stock?
Сколько разморозок выдерживает?
Какой у него коэфиециент экстинкции?
guest: ммм , 04.03.2013 21:23
--Сколько может прожить в DMSO 200 mM stock?
Зависит от количества растворенного в растворе кислорода. Если его нет вообще, то дооолго - годы. А если как обычно ну думаю от недель до полугода.

--Сколько разморозок выдерживает?
По хрену ему разморозки тем более в ДМСО.

Экстинция.
Полагаю около 20 для 1мМ раствора при длине волны 331нм
и около 4 для 245нм
-Ъ-, 05.03.2013 00:25
Ходячая энциклопедия cool.gif
guest: ммм , 05.03.2013 09:01
Всего лишь спецификация от сигмы. smile.gif
mesentsev, 05.03.2013 12:56
> Полагаю около 20 для 1мМ раствора при длине волны 331нм
> и около 4 для 245нм

Извините, а Вас не смущает, чио раствор желтого цвета?
mesentsev, 05.03.2013 12:58
> Всего лишь спецификация от сигмы.

А что Вы имеете в виду под "спецификацией"?
mesentsev, 05.03.2013 13:02
> Ходячая энциклопедия

Хорошо, можно поставить вопрос по-другому.
Где в Интернете можно посмотреть спектры поглощения?
Неужели нет базы данной, аналогичной той, что есть по MS?
genseq, 05.03.2013 14:27

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guest: ммм , 05.03.2013 18:06
--Извините, а Вас не смущает, чио раствор желтого цвета?

Почему оно должно меня волновать я же вашего раствора не вижу.

--А что Вы имеете в виду под "спецификацией"?
http://www.sigmaaldrich.com/Graphics/COfAI...__SIGMA____.pdf

-Где в Интернете можно посмотреть спектры поглощения?
Неужели нет базы данной, аналогичной той, что есть по MS?

А где вы видели базы MS низкомолекулярных в-в в открытом доступе, они все платные если есть.

Что касается базы UV-спектров, то их нет и быть не может, в отличии скажем от IR-спектров, потому что UV спектры малоинформативны и не могут служить для идентификации в-ва или подтверждения его строения.
guest: ммм , 05.03.2013 18:08
Желтизна раствора может свидетельствовать появление коллоидной(или растворенной) серы в растворе, что говорит об окислении в-ва.
mesentsev, 06.03.2013 10:38
для guest: ммм:

Я понял. что Вы имели ввиду. Спасибо Вам за спецификацию.
mesentsev, 06.03.2013 10:46
В DMSO спектр имеет пик на 380 nm и плечо около 400 нм.
В старом препарате плечо подросло, т.е. таки да, - видимо, сера.

А почему 331 нм указан для измерения экстинкции я не понимаю - ну, нет у 4-тиоуридина поглощения в этой части спектра: ни в DMSO, ни в ацетонитриле.
mesentsev, 06.03.2013 10:47
Спасибо.
mesentsev, 06.03.2013 10:50
Это то, что я записал в DMSO.
Что-то не так?


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