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 Problem list:

  • No of bags in a box / 15ml, 50ml?


Mol.biology // Next-generation sequencing

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Содержание


NB! list for Autom. group. If you want to organize for your oun group; otrganize a page of your group; f.e., and as a subpage


[править] E-lager

itemshould be in reserve
(minimum)
to order
glovesXLfor all sizes: 4for all sizes: 4
M
L
S
ethanol (p.A.) 1 full 2.5 L bottle 1 bottle
scalpels 3 boxes5 boxes
tubes0.5 ml1 unopened bag1 box
1.5 ml1 unopened bag1 box
2 ml1 unopened bag1 box
15 ml5 unopened bags1 box (? bags)
50 ml5 unopened bags1 box (? bags)
8 tube-strips  1 unopened box2 boxes
caps for stripes  1 unopened box1 box



[править] Other suppliers

[править] Plastic

itemcompany, cat.no.should be in reserveto order
DNA LoBind tube, 250x per box0.5mlEppendorf, #0030 108.0355 boxes15 boxes
1.5mlEppendorf, #0030 108.0513 boxes10 boxes
"MAXYMum Recovery" Filter Tips, Racked, Pre-Sterilized1000µl, 1000x per boxVWR, #732-08632 boxes5 boxes
200µl, 960x per boxVWR, #732-06554 boxes10 boxes
20µl, 960x per boxVWR, #732-06524 boxes10 boxes
2µl, 960x per boxVWR, #732-06492 boxes5 boxes
TubesQubit-tubes, 0.5ml, 1000xVWR, #10011-830??
Reaktiongefasse ohne deckel, 2ml, 1000xA.Hartenstein GmBH, #RK20??
RT-PCR tubes, flat cap, 100µl48x plate???
8-strip???
individual tubes???
Saran-Film300 m x 300 mm, 11,5 ?mA.Hartenstein GmBH, #SA30??



[править] Kits, reagents

itemcompany, cat.no.should be in reserveto order
Spin-columnsQIAquick PCR Purification Kit (250)Qiagen, #28106??
QIAquick Gel Extraction Kit (250)Qiagen, #28706??
MinElute Reaction Cleanup Kit (250)Qiagen, #28206??
RNeasy MinElute Cleanup Kit (50)Qiagen, #74204??
Quant-iT*dsDNA BR Assay Kit, 500 assaysInvitrogen, #Q32853??
dsDNA HS Assay Kit, 500 assaysInvitrogen, #Q32854??
RNA Assay Kit, 500 assaysInvitrogen, #Q32855??
RNA BR Assay Kit, 500 assaysInvitrogen, #Q10211??
BuffersNuclease-free Water, 500ml
aliquotes: ?x?0ml, store at
Ambion, #AM9930??
1 M Tris, pH 7.0, 100ml
aliquotes: ?x?0ml, store at
Ambion, #AM9850G??
1 M Tris, pH 7.0, 100ml
aliquotes: ?x?0ml, store at
Ambion, #AM9855G??
0.5M EDTA, pH 8.0, 100ml
aliquotes: ?x?0ml, store at
Ambion, #AM9260G??
DNA Typing Grade 50X TAE Buffer, 1L
aliquotes: ?x?0ml, store at
Invitrogen, #24710-030??
Low Range Ultra Agarose, 125gBio-Rad, #161-3107??
* Quant-iT aliquotes: 20x63µl Component A, 20x250µl Components C and D, ? Components B





[править] Enzymes

itemcompany, cat.no.should be in reserveto order
polymerasesDNA Pol. I (E. coli), 2500u, 10u/µl
aliquotes: 5x50µl
NEB, M0209L??
DNA Pol. I, Klenow large fragment, 1000u, 5u/µl
aliquotes: 5x40µl
NEB, M0210L??
Klenow Fragment (3'->5' exo–), 1000u, 5u/µl
aliquotes: 5x40µl
NEB, M0212L??
T4 DNA Pol., 750u, 3u/µl
aliquotes: 5x50µl
NEB, M0203L??
Phusion High-Fidelity PCR Master Mix with HF Buffer, 10x 1.25ml
aliquote each: 5x250µl
NEB, F-531L??
NA modification enzymesQuick Ligation kit
aliquotes: 3x50µl enzyme, 8x250µl buffer
NEB, M2200L??
T4 Polynucleotide Kinase, 2500u, 10u/µl
aliquotes: 5x50µl
NEB, M0201L??
RNase H, 1250u, 5u/µl
aliquotes: 5x50µl
NEB, M0297L??
T7 Exonuclease, 5000u, 10u/µl
aliquotes: 10x50µl
NEB, M0263L??
Buffers5x Second-Strand Buffer, 0.5ml
aliquotes: 10x50µl
Invitrogen, #10812-014??

TURBO™ DNase (2 U/µl) Ambion AM2238

www.epicentre.com Plasmid-Safe™ ATP-Dependent DNase Epicentre E3105K $200


|- align='center' | class='highlight' align='left'|, 1000u, 5u/µl

aliquotes: 5x40µl

||NEB, #||?||?



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